Tag

Part:BBa_M36144:Experience

Designed by: Tatyanna Dadabbo & Richard Hojel & Soraya Karimi   Group: Stanford BIOE44 - S11   (2015-10-22)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_M36144

User Reviews

UNIQ6af731181abc5c60-partinfo-00000000-QINU UNIQ6af731181abc5c60-partinfo-00000001-QINU

12/04/15

This His tag was codon optimized for E.Coli and specific for the EP-B2 protein. It's a marker for the EP-B2 protein that can be used for identification. Another purpose of the His tag is protein purification, specifically The EP-B2 protein. When planning our experiments, we were going to do a Western Blot, however, we ended up not needing to do a Western Blot, because our other assays provided sufficient results. Since we did not do a Western Blot the His tag was not actually a necessary part of the construct. If we end up doing follow-up experiments this His tag will be necessary.